Journal: eLife

Article Title: Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics

doi: 10.7554/eLife.83477

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , AlphaScreen SureFire pERK 1/2 (Thr202/Tyr204) Assay Kits , PerkinElmer , , .

Techniques: Purification, Expressing, Plasmid Preparation, Recombinant, Dominant Negative Mutation, Amplified Luminescent Proximity Homogenous Assay, Software

Journal: BMC Cancer

Article Title: SC-III3, a novel scopoletin derivative, induces cytotoxicity in hepatocellular cancer cells through oxidative DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation

doi: 10.1186/1471-2407-14-987

Figure Lengend Snippet: Effect of SC-III3 on DNA damage and ATM/ATR pathways. (A) HepG2 cells were treated with SC-III3 for 24 hours. Western blot analysis of p-H2AX, total H2AX, p-Chk1 (Ser280) and p-Chk1 (Ser296) response to SC-III3. (B) HepG2 cells were treated with SC-III3 for 24 hours. Western blot analyses of the ATM/ATR signaling related proteins in HepG2 cells. (C) HepG2 cells were treated with 1 μM SC-III3 for different times as indicated. Western blot analyses of p-ATM, p-ATR, p-Chk1, p-Chk2, Cdc25C, p-CDK2, p53, and p21 in HepG2 cells. These experiments were done in triplicates.

Article Snippet: Antibodies against p-ATM (Ser1981), ATM, p-ATR (Ser428), ATR, p-Chk1 (Ser345), p-Chk1 (Ser280), p-Chk1 (Ser296), Chk1, p-Cdk2 (Tyr15), p53, p21 were purchased from Cell Signaling Technology (Danvers, MA). p-Chk2 (Thr68), Chk2, Cdc25A, p-H2AX(Ser139), H2AX antibodies were purchased from EnoGene Biotech (Nanjing, China), GAPDH monoclonal antibodies were purchased from Kangchen Bio-tech (Shanghai, China); Cdk2, cyclinA, cyclinE, cyclinB, Bax, Bcl-2 monoclonal antibodies were purchased from Bioworld (Georgia, USA).

Techniques: Western Blot

Journal: BMC Cancer

Article Title: SC-III3, a novel scopoletin derivative, induces cytotoxicity in hepatocellular cancer cells through oxidative DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation

doi: 10.1186/1471-2407-14-987

Figure Lengend Snippet: Role of ATM/Chk1/Chk2 in SC-III3-mediated S cell cycle arrest. (A, B) HepG2 cells were exposed to 1 μM SC-III3 for 24 h with or without adding of 10 μM Ku55933 or 0.3 μM UCN-01. Then, cells were analyzed for cell cycle distribution by flow cytometry. Cells in G0/G1, S, and G2/M phases were quantified and presented. (C) HepG2 cells were exposed to 1 μM SC-III3 with or without adding of 10 μM Ku55933 for 24 h. Then, western blot analyses of p-ATM, p-ATR, p-Chk1, p-Chk2, Cdc25C, p-CDK2, p53, and p21 in HepG2 cells. (D) HepG2 cells were exposed to 1 μM SC-III3 with or without adding of 0.3 μM UCN-01 for 24 h. Then, western blot analyses of p-Chk1, p-Chk2, p-Cdk2, and Cdc25A in HepG2 cells. The Data were presented as mean ± SEM for three separate experiments. The difference were significant at ** p < 0.01 compared with SC-III3 (1 μM) and ## p < 0.01 compared with SC-III3 (0 μM).

Article Snippet: Antibodies against p-ATM (Ser1981), ATM, p-ATR (Ser428), ATR, p-Chk1 (Ser345), p-Chk1 (Ser280), p-Chk1 (Ser296), Chk1, p-Cdk2 (Tyr15), p53, p21 were purchased from Cell Signaling Technology (Danvers, MA). p-Chk2 (Thr68), Chk2, Cdc25A, p-H2AX(Ser139), H2AX antibodies were purchased from EnoGene Biotech (Nanjing, China), GAPDH monoclonal antibodies were purchased from Kangchen Bio-tech (Shanghai, China); Cdk2, cyclinA, cyclinE, cyclinB, Bax, Bcl-2 monoclonal antibodies were purchased from Bioworld (Georgia, USA).

Techniques: Flow Cytometry, Western Blot